P238 DIFFERENTIAL EXPRESSION OF LEPTIN AND LEPTIN'S RECEPTOR ISOFORM (Ob-Rb) mRNA BETWEEN ADVANCED AND MINIMALLY AFFECTED OSTEOARTHRITIC CARTILAGE

Detection of leptin and leptin receptor mRNA expression in the bovine t

Differential Immunohistochemical Expression Pattern of Galectin-3 in Normal and Osteoarthritic Human Articular Cartilage

Background: Previous studies have shown that Galectin-3, a member of lectin family, is expressed in developing cartilage in mouse embryo and also in growth plate of long bones.   Objective: In the present work, the expression pattern of Galectin-3 in normal and various grades of osteoarthritic (OA) human articular cartilage has been studied.   Methods: Using immunohistochemistry and standard we...

Leptin receptor mRNA expression in Taleshi ram gonads

Leptin is secreted mainly by fat, which is involved in energy metabolism and reproduction. Leptin and itsreceptor (Ob-R) have been detected in human spermatozoa and testis, thus it can be concluded leptin involves in male physiology. The goal of this study was determination of the presence of leptin receptor mRNA in the ram testis, epididymis and spermatozoa by reverse transcription polymerase ...

Expression of leptin and leptin receptor transcript in ovine corpus luteum

Background: Leptin, the product of the obesity (ob) gene, acts as a signaling adipokine for modulating food intake, energy metabolism and reproductive functions in mammals. Leptin’s effects on the reproductive system at various levels of the hypothalamic-pituitary-gonadal axis have been established. Moreover, the direct and local effect of leptin on bovine oocyte maturation and corpus luteum fu...

Identification and expression cloning of a leptin receptor, OB-R

The ob gene product, leptin, is an important circulating signal for the regulation of body weight. To identify high affinity leptin-binding sites, we generated a series of leptin-alkaline phosphatase (AP) fusion proteins as well as [125I]leptin. After a binding survey of cell lines and tissues, we identified leptin-binding sites in the mouse choroid plexus. A cDNA expression library was prepare...